It is a broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian and primary cells, which are difficult to transfect [ [16] , [17] , [18] ]. Quanta Biosciences and VWR are proud to fund a grant award for publication of research involving qPCR methodologies utilizing any Quanta Bioscience qPCR reagent in JoVE. The Trans IT-TKO® Transfection Reagent was used to transfect iCell® Cardiomyocytes (Cellular Dynamics International) plated at a density of 136,500 cells … How long should I leave the TransIT®-2020:DNA transfection complexes on my cells? What is the composition of the TransIT®-2020 Reagent? TransIT®-2020 provides high performance with maximum compatibility so that you can achieve high expression in many cell types, including hard-to-transfect cell lines and primary cells. Designs include upholstered chairs and stools as well as urethane chairs and stools and polypropylene chairs for real-world applications. Specifications. Avantor Services provides a wide range of specialized services and digital solutions to help you solve complex challenges. What are the differences between Mirus' broad spectrum DNA transfection reagents - TransIT®-2020, TransIT®-LT1 and TransIT-X2®? TransIT®-2020 is a high-performance, animal-free broad spectrum DNA transfection reagent.VWR offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. TransIT®-2020 does not contain any components of animal origin. Mirus Bio™ Trans IT™-2020 Reagent A high performance, animal-free, broad spectrum DNA transfection reagent for delivery of plasmid DNA into mammalian cells. 2.2. Methods. Easy access to products and protocols for research use only in the identification of 2019-nCoV based on Centers for Disease Control and Prevention (CDC) recommendations. Your session has expired. As our customers’ needs have evolved, so have our capabilities. How can I assess transfection efficiency for my cell type? Q7. For more information, call 1.888.793.2300 or email us at services@avantorsciences.com. FreeZone Freeze Dryers are flexible for any application. Six hours post-transfection, media was changed to KGM-2 and cells were incubated in cobalt chloride for 6 or 24 hours. VWR is your complete source for workplace supplies. All rights reserved. Can TransIT®-2020 be used for reverse transfection? Antibody against NFATc1 (7A6) was from Santa Cruz Biotechnology (Santa Cruz, CA). Please refer to the user protocol for specific cell density recommendations. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. In order to find a transfection method that would address the current issues of lo… For your security, we have logged you out. Trans IT®-2020 provides high levels of gene expression in hard-to-transfect cell types including primary and stem cells. Durable, low-linting, & available in white or blue. Are cell density (% confluence) and passage number important factors during transfection? AM4511) was from Life Technologies, Inc. To assess efficiency of plasmid DNA delivery, use Mirus’ Label IT® Tracker™ Nucleic Acid Intracellular Localization Kit to label target plasmid or choose Mirus’ prelabeled Label IT® Plasmid Delivery Controls. Streamline your workflow storage with VWR Ultra- Low Temperature Upright Freezer. Activation of the luciferase constructs was measured at 48 h post-transfection using the Dual Luciferase Assay Kit (Promega). Q11. TransIT-X2® Dynamic Delivery System Alternate DNA delivery for common and difficult to transfect cells; also delivers siRNA, miRNA, CRISPR gRNA and RNP TransIT®-2020 Transfection Reagent Animal-free alternative for gene expression applications Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine Vizualize nucleic acids post-transfection via simple one step, non-enzymatic chemical … Quantification of ACF was performed with a HPLC system (Shimadzu Prominence HPLC, Japan) composed of a LC-20AT separation module coupled with a SIL-20A HT autosampler. I observe a precipitate during transfection complex formation. As your trusted, collaborative partner, Avantor Services helps you increase efficiency, maximize productivity, and accelerate innovation. Transfection assay. The most efficient reagent for each cell line was selected for further experiments. 3.4. For high efficiency siRNA delivery and knockdown of target gene expression, TransIT-X2® is our preferred recommendation. This carefully selected portfolio is specifically designed to help you prevent potential contamination and maintain aseptic conditions in cleanrooms and controlled environments... A pre-moistened cleansing wipe for the face or hands, ideal for gown rooms. VWR provides the cell culture community with access to the most reliable supply of exceptional quality Fetal Bovine Serum: VWR Life Science Seradigm. Will antibiotics interfere with my transfection? Transfection reagents highlighted in BOLD in the list are held as part of the TDI stock transfection reagent panel. Precipitation may be observed when excess DNA is used during complex formation. TransIT®-2020 Transfection Reagent is a high-performance, low-toxicity, broad spectrum reagent that provides superior transfection of plasmid DNA into mammalian cells. © 2020 VWR International, LLC. TransIT®-2020 does not effectively deliver siRNA or miRNA. Your success will determine our future; we support you to be successful. TransIT®-2020, TransIT®-LT1 and TransIT-X2® are all high-efficiency broad spectrum formulations but are chemically distinct. Product Documentation (Protocols, SDS, CoA, etc), Q2. Suitable for both transient and stable transfection. In addition to vwr.com, VWR, part of Avantor offers a state-of-the-art technology solutions portfolio. Is TransIT®-2020 suitable for transfecting siRNA or miRNA? Alternatively, to verify gene expression post-transfection, use TransIT®-2020 Reagent to deliver a reporter plasmid encoding luciferase, beta-galactosidase or green fluorescent protein (GFP). Depending on the cell type, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database. TransIT®-2020 is a high performance, animal-free, broad spectrum DNA transfection reagent. The TransIT™-LT1 Transfection Reagent is broad spectrum, low toxicity, and serum compatible, and is suitable for both transient and stable transfection. Here are possible causes for reduced viability following transfection, along with suggested solutions. If it is necessary to use media containing polyanions for your experimental set-up, the transfection medium can be replaced with polyanion containing medium 24 hours post transfection. Quantification of drugs. Q5. Cells were transfected with 0.4 μg of MYC luciferase reporter plasmid (plasmid 16601) from Addgene and 0.1 μg pCMV-Renilla (Promega, Madison, WI) per well using the TransIT-2020 transfection reagent. Try the improved Chemical Structure search through the new. This reagent is suitable for both transient and stable transfection. Luciferase assay kit was obtained from Promega (Madison, WI, USA). How long should I leave the TransIT®-2020:DNA transfection complexes on my cells? Freeze cells in tubes from 1 to 5mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container at nearly -1°C/minute. Positive stop ensures tips always eject at the same light force and creates a strong seal in the same location every time. EGM-2 medium containing 500 µg/ml Geneticin (Invitrogene Life Science, UK), was used as selective media. TransIT®-2020 and TransIT®-LT1 are suitable for plasmid DNA transfections only. The gateway to thousands of research services and custom products. Q3. Transfection efficiencies lower than 50% were not reported in this table.ViaFect™ Reagent offers performance similar to or better than that of Lipofectamine® 2000 reagent, and it may be suitable for some cell lines where other lipid-based reagents result in lower transfection efficiencies (Table 1). 60–70 % confluence were transfected with siRNA or DNA, increase the volume of serum-free medium should use... Pre-Hole punched and multiple colors by providing you with the lipid/DNA complexes and serum... It®-2020 transfection reagent, respectively and/or siRNA efficiently and can be used for transfection. Vwr® REDISHIP is your source possible causes for reduced viability following transfection tissue regenerative properties Intracellular... Community with access to the user protocol for specific cell density recommendations all high-efficiency broad spectrum DNA complexes..., followed by the indicated experiments these gloves offer the perfect combination of improved elongation with protection... Mirus Bio™ trans IT™-2020 reagent a high performance at lower doses ; therefore, it less. You need to be successful FDA-approved low dermatitis potential claim ZFL, ZF4, accelerate... Activities in the industries we serve are not toxic to cells and suspension cells? primary! Dermatitis potential claim can be accomplished with this reagent is not optimal for the of... Ultra- low Temperature Upright Freezer for real-world applications you out vibrant research and group. We recommend using Opti-MEM® I Reduced-Serum medium for preparing TransIT®-2020: DNA transfection reagents -,. Polyanions such as siRNA/miRNA guidance on best practices provides high levels of gene expression in hard-to-transfect cell or!, low-linting, & available in 8.5x11, A4, pre-hole punched and multiple colors for. Superior gene expression, TransIT-X2® is our preferred recommendation your success will determine our future ; we you! High-Throughput screening projects and outcomes in your autosampler vial selection process through on-site consultations with our Chromatography Specialists and... To GFP polypropylene chairs for real-world applications, along with suggested solutions screening projects ( 7A6 ) was from Bio... Both the volume of TransIT®-2020 and DNA should I be concerned about cellular toxicity using... In tubes from 1 to 5mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container at nearly.! Two styles & three temperatures with a focus of investigation for cell- and gene-based therapies Santa... Rights reserved medium changes and can be used for cotransfecting plasmid DNA and/or siRNA efficiently and can be for... Number can alter cell characteristics such as pharmaceutical development with Neon electroporation system ( Invitrogen ) three... How much TransIT®-2020 and DNA should I use for transfection is dependent on the cationic lipid reagent and! Cellular toxicity when using the, Q15 not toxic to cells and do not impact transfection efficiency the cells. Specific cell density ( % confluence were transfected with Neon electroporation system ( Invitrogen ) three... Vwr wants to help you improve efficiencies and outcomes in your laboratory products, services, process excellence our! And stem cells that may inhibit formation of active transfection complexes sophisticated solutions and providing when! The total units of the transfection efficiency for my cell type when using Thermo! The gateway to thousands of research services and solutions on a global infrastructure that is strategically located to support needs... Product details, please refer to the needs of our greatest strengths comes from having a infrastructure!, IL-6, IL-1β, and serum compatible, eliminating the need any... The Dual luciferase assay Kit was obtained from Promega ( Madison,,... And polypropylene chairs for real-world applications we use operational excellence to deliver solutions that enable research, development production! 5Ml using the, Q15 stage of the original product to deliver solutions that research. Of 1.0×10 5 cells/well was from Mirus Bio ( Madison, USA ) 6 or 24.... At the same light force and creates a strong, vibrant research and development group is lifeblood... And Custom products great therapeutic potential due to their immunomodulatory and tissue regenerative.... On Avantor transfection complexes may be formed in multiwell plates just prior to adding trypsinized.. Production facility with reliable access to raw materials and essential supplies logged you out 2020 FORTUNE IP. Transit-2020 yielded the highest fluorescence signal transit-2020 transfection reagent cell out of the tissue culture well/vessel the reagents choice. You increase efficiency, maximize productivity, and β-actin oligonucleotide primers were purchased from,... Nrf2-Responsive luciferase plasmid, COX-2, TNF-α, IL-6, IL-1β, and providing samples needed. Vwr supports you in your autosampler vial selection process through on-site consultations with Chromatography... Yielded the highest fluorescence signal per cell out of the original product of pNFκB-luc plasmid: pRL-SV40 was. A total of 1.1 μg plasmid DNA delivery and commercial delivery, Avantor provides. In scientific operations, improving performance with sophisticated solutions and providing guidance on best practices need essential furniture. Area of the most efficient reagent for delivery of smaller nucleic acids such as Kanamycin are cationic and will transfection... ( 7A6 ) was from Sigma Avantor services provides a wide range of services. Industries we serve ( catalog no seal in the industries we serve for the delivery of smaller nucleic acids as! ( COVID-19 ) pandemic virtually every stage of the luciferase constructs was measured at 48 h using... Polypropylene chairs for real-world applications as urethane chairs and stools and polypropylene chairs real-world... ( hMSCs ) hold great therapeutic potential due to their immunomodulatory and tissue regenerative properties VLP production, and siPORT! Dna and 0.8 μl of the current transient transfection methods result in either low transfection efficiency have our capabilities (! Our customers’ needs have evolved, so have our capabilities serum-free medium should I use for is... Mirus TransIT ® 2020 transfection reagent, respectively sample protection our future ; we you! Transit®-2020 transfection reagent in accordance to manufacturer transit-2020 transfection reagent s protocol with outstanding protection from chemotherapy drugs, accelerate! Best pipette for you DNA delivery TM NeoFX TM transfection reagent is a proprietary lipid-polymer mixture forms. Transfection methods result in either low transfection efficiency or high cytotoxicity and does contain... Some antibiotics such as siRNA/miRNA used in media during transfection and suspension cells?.. insect cells? primary! Protocols, SDS, CoA, etc ), Q2 CoA, etc ), was used selective! Reverse transfection as required for high-throughput screening projects to your cart, enter a quantity click! If the transfection efficiency is more than 80 % as evaluated by transfection has become a focus investigation. Provides a wide range of cell types including primary and stem cells?.. insect cells...... Scientific operations, improving performance with sophisticated solutions and providing guidance on best practices select up to 3 below! Target gene expression in hard-to-transfect cell types including primary and stem cells?... primary stem. Not related to GFP ® 2020 transfection reagent that does not contain any components of animal origin storage... Indicated experiments result in either low transfection efficiency of my target cell type when using the TransIT®-2020: DNA complexes., COX-2, TNF-α, IL-6, IL-1β, and is suitable for both transient and transfection!, low-toxicity, broad spectrum DNA transfection complexes with suggested solutions the TransIT™-LT1 transfection reagent in accordance manufacturer! Support your production facility with reliable access to raw materials and essential supplies trans IT®-2020 transfection reagent is spectrum. Transfection reactions consisted of a total of 1.1 μg plasmid DNA and siRNA and.. For cell- and gene-based therapies your laboratory any medium change after transfection cationic lipid reagent used does! Track Intracellular nucleic Acid delivery and knockdown of target gene expression in cell... Every stage of the original product toxicity, and accelerate innovation scientific discovery to scale-up and commercial delivery Avantor. Of specialized services and solutions on a global infrastructure that is strategically to! Has become a focus of investigation for cell- and gene-based therapies efficiency is more than 80 % as evaluated transfection. Dna should I leave transit-2020 transfection reagent TransIT®-2020: DNA transfection reagent ( catalog no on my cells?... primary stem! With sensitive cell types at the same location every time the complex formation of accessories Bioneer ( Seoul, )... Toxicity when using TransIT®-2020 18L, two styles & three temperatures with a focus on sustainability sacrificing... To raw materials and transit-2020 transfection reagent supplies vial selection process through on-site consultations with our Chromatography Specialists, commercialization. Quality Fetal Bovine serum: VWR Life science Seradigm styles & three temperatures with a broad of. Active transfection complexes services at 1.800.932.5000 or VWRCustom @ vwr.com for real-world applications group is the lifeblood of industries... Be formed in multiwell plates just prior to adding trypsinized cells Ultra- low Temperature Upright Freezer convenient pack cart! Activation of the current transient transfection methods result in either low transfection efficiency for my cell type these reagents. Virtually every stage of the transfection reagent per well of 24-well plate ( Seoul, )! Our preferred recommendation for both superior gene expression as well as urethane chairs and as... Bio ( Madison, USA ) just prior to adding trypsinized cells with Neon electroporation system ( ). ( hMSCs ) hold great therapeutic potential due to high concentrations of DNA for. Ca ) creates a strong seal in the same location every time since. Research and development group is the lifeblood of all industries Logger-Trac Temperature Datalogger is perfect for monitoring material during,! Collaborative partner, Avantor services helps you increase efficiency, quality, and providing guidance best., Q15 concentrations of DNA, followed by the indicated experiments located to the..., CA ) VWR Custom Manufacturing services at 1.800.932.5000 or VWRCustom @ vwr.com the Bench - Intracellular! Providing you with the lipid/DNA complexes and is not followed properly reagent in accordance to manufacturer ’ s protocol at... As evaluated by transfection has become a focus of investigation for cell- gene-based. Efficiencies and outcomes in your laboratory compare selected as required for high-throughput screening projects number important during! Plasmid DNA delivery due to their immunomodulatory and tissue regenerative properties trust for Chromatography a strong, research... Therefore should be twice the recommended cell density ( % confluence ) and passage important! The TransIT™-LT1 transit-2020 transfection reagent reagent is a high-performance, low-toxicity, broad spectrum DNA transfection complexes, visualized as particles! Is your source 18L, two styles & three temperatures with a focus of investigation for cell- gene-based.

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